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pcdna3 1 flag hdac1  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 1 flag hdac1
    Pcdna3 1 Flag Hdac1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+hdac1/HDAC1+Flag+(Plasmid+%2313820)/us12570957-1697-22-25
    Average 93 stars, based on 69 article reviews
    pcdna3 1 flag hdac1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Twist1 and Twist2 induce human macrophage memory upon Chronic Innate Receptor Treatment by HDAC-Mediated Deacetylation of Cytokine Promoters
    Article Snippet: Protein expression analysis Western blot analysis and intracellular flow cytometry was performed as in ( 29 ) using antibodies to Twist1/Twist2 (Twist2C1a), HDAC1 (10E2), HDAC3 (A-3) (Santa Cruz), NOD2 (Cayman) or GAPDH (6C5; EMD Millipore).

    Transfection:

    Article Title: Twist1 and Twist2 induce human macrophage memory upon Chronic Innate Receptor Treatment by HDAC-Mediated Deacetylation of Cytokine Promoters
    Article Snippet: Western blot analysis and intracellular flow cytometry was performed as in ( 29 ) using antibodies to Twist1/Twist2 (Twist2C1a), HDAC1 (10E2), HDAC3 (A-3) (Santa Cruz), NOD2 (Cayman) or GAPDH (6C5; EMD Millipore). .. Primary human MDMs or mouse BMDMs were transfected with 100nM scrambled or ON-TARGETplus SMARTpool siRNA against Twist1, Twist2, HDAC1 or HDAC3 (Dharmacon, Lafayette, CO) (4 pooled siRNAs for each gene), or with 3.0 μg pcDNA3.1-HDAC1 (#13820) or pcDNA3.1-HDAC3 (#13819) (kindly deposited to Addgene by Eric Verdin) ( 30 ) or empty vector using Amaxa nucleofector technology (Lonza Bioscience, Walkersville, MD). .. Western blot analysis and intracellular flow cytometry was performed as in ( 29 ) using antibodies to Twist1/Twist2 (Twist2C1a), HDAC1 (10E2), HDAC3 (A-3) (Santa Cruz), NOD2 (Cayman) or GAPDH (6C5; EMD Millipore).

    Plasmid Preparation:

    Article Title: Twist1 and Twist2 induce human macrophage memory upon Chronic Innate Receptor Treatment by HDAC-Mediated Deacetylation of Cytokine Promoters
    Article Snippet: Western blot analysis and intracellular flow cytometry was performed as in ( 29 ) using antibodies to Twist1/Twist2 (Twist2C1a), HDAC1 (10E2), HDAC3 (A-3) (Santa Cruz), NOD2 (Cayman) or GAPDH (6C5; EMD Millipore). .. Primary human MDMs or mouse BMDMs were transfected with 100nM scrambled or ON-TARGETplus SMARTpool siRNA against Twist1, Twist2, HDAC1 or HDAC3 (Dharmacon, Lafayette, CO) (4 pooled siRNAs for each gene), or with 3.0 μg pcDNA3.1-HDAC1 (#13820) or pcDNA3.1-HDAC3 (#13819) (kindly deposited to Addgene by Eric Verdin) ( 30 ) or empty vector using Amaxa nucleofector technology (Lonza Bioscience, Walkersville, MD). .. Western blot analysis and intracellular flow cytometry was performed as in ( 29 ) using antibodies to Twist1/Twist2 (Twist2C1a), HDAC1 (10E2), HDAC3 (A-3) (Santa Cruz), NOD2 (Cayman) or GAPDH (6C5; EMD Millipore).

    Article Title: Identification of an Intrinsic Determinant Critical for Maspin Subcellular Localization and Function
    Article Snippet: The amplified fragment was sub-cloned into pEGFP-N1 mammalian expression vector from Clontech (Mountain View, CA). .. For the construction of HDAC1-red fluorescent protein (RFP) chimera, mRFP was PCR amplified from pmRFP1-N1 vector and inserted into pcDNA3.1-HDAC1 (Addgene repository, plasmid 13820) and ligated using EcoRI. .. For this reason, an additional N-terminal EcoRI site had to be eliminated first by site-directed mutagenesis (QuikChange Lightning Site-Directed Mutagenesis Kit, Agilent Technologies, Santa Clara, CA).

    Polymerase Chain Reaction:

    Article Title: Identification of an Intrinsic Determinant Critical for Maspin Subcellular Localization and Function
    Article Snippet: The amplified fragment was sub-cloned into pEGFP-N1 mammalian expression vector from Clontech (Mountain View, CA). .. For the construction of HDAC1-red fluorescent protein (RFP) chimera, mRFP was PCR amplified from pmRFP1-N1 vector and inserted into pcDNA3.1-HDAC1 (Addgene repository, plasmid 13820) and ligated using EcoRI. .. For this reason, an additional N-terminal EcoRI site had to be eliminated first by site-directed mutagenesis (QuikChange Lightning Site-Directed Mutagenesis Kit, Agilent Technologies, Santa Clara, CA).

    Amplification:

    Article Title: Identification of an Intrinsic Determinant Critical for Maspin Subcellular Localization and Function
    Article Snippet: The amplified fragment was sub-cloned into pEGFP-N1 mammalian expression vector from Clontech (Mountain View, CA). .. For the construction of HDAC1-red fluorescent protein (RFP) chimera, mRFP was PCR amplified from pmRFP1-N1 vector and inserted into pcDNA3.1-HDAC1 (Addgene repository, plasmid 13820) and ligated using EcoRI. .. For this reason, an additional N-terminal EcoRI site had to be eliminated first by site-directed mutagenesis (QuikChange Lightning Site-Directed Mutagenesis Kit, Agilent Technologies, Santa Clara, CA).



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